Non-adenoviral gene product-based complementing cells for adenoviral vectors
AssigneeGenVec, Inc.
InventorsDouglas E. Brough, Jason G. D. Gall, Imre Kovesdi
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Abstract
The invention provides cells and methods of using the cells for the propagation of replication-deficient adenoviral vectors. The cells comprise at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.
Claims37
A human retinal cell comprising at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of one or more regions of an adenoviral genome selected from the group consisting of the E1, E2A, and E4 regions so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the E1, E2A, and/or E4 regions when present in the human retinal cell.
A lung carcinoma cell comprising at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of the E1 region of an adenoviral genome and a deficiency in one or more essential gene functions in either or both of the E2A region and the E4 region the adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the E1 region and either or both of the E2A region and the E4 region when present in the lung carcinoma cell.
A cell having a cellular genome comprising at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of the E4 region of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the E4 region when present in the cell.
Dependencies are inferred from the claim text.