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US 6,676,819 B1Granted
Methods and apparatus for automatic on-line multi-dimensional electrophoresis
OwnerYaoqing Diana Liu, James Jianmin Baoinventors
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Abstract
The invention provides a method of performing one- and two-dimensional electrophoresis with or without gel in an automated way. The two dimensional electrophoresis can be performed either separately or continuously. Both electrokinetic and hydrodynamic forces can be used to facilitate sample transfer from the first to the second dimension. The samples can be detected on-line by using common detectors like UV-Vis, laser induced fluorescence (LIF), and mass spectrometry (MS).
Claims30
A system for performing two-dimensional electrophoresis, comprising:
- a first electrophoresis device having
- a first cathode,
- a first anode,
- a substrate having a top surface, a bottom surface, side surfaces, and at least one channel disposed within the substrate for transporting fluid during electrophoresis, and
- a substantially planar top cover on the top surface of the substrate to a sealed channel in the substrate;
- and
- a second electrophoresis device having
- a second cathode,
- a second anode,
- a buffer reservoir, and
- a plurality of capillaries, each of the capillaries having a longitudinal axis which is positioned angularly relative to the direction of electric current flow from the anode to the cathode of the first electrophoresis device, a proximal end capable of being electrically coupled to the channel within the substrate, and a distal end in contact with the buffer reservoir of the second electrophoresis device,
- wherein the planar top cover is aligned to be coplanar with the substrate of the first electrophoresis device and contains perforated openings for access of the capillaries to the sealed channel.
9 more elements
A system for performing two-dimensional electrophoresis, comprising:
- a first electrophoresis device having
- a first cathode,
- a first anode,
- a substrate having a top surface, a bottom surface, side surfaces, and at least one channel disposed within the substrate, the channel being adapted for transporting fluid during electrophoresis, and
- a substantially planar top cover on the top surface of the substrate to form a sealed channel in the substrate;
- and
- a second electrophoresis device having
- a second cathode,
- a second anode,
- a buffer reservoir, and
- a plurality of capillaries, each of the capillaries having a longitudinal axis which is positioned angularly relative to the direction of electric current flow from the anode to the cathode of the first electrophoresis device, a proximal end coupled to the sealed channel within the substrate via a metal tubing tip configured for pinching through the top cover to establish fluidic communication between the capillaries and the channel, and a distal end in contact with the buffer reservoir of the second electrophoresis device.
8 more elements
Dependencies are inferred from the claim text.