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US 6,674,525 B2Granted
Split focusing cytometer
AssigneeMicronics, Inc.
InventorsRonald Bardell, Bernhard H. Weigl, C. Frederick Battrell
Life of this patent
Estimating the term…
Abstract
A microcytometer which combines lysing and cytometry into a unified system that achieves blood lysis and white blood cell count in a single device. The device focuses the white cells into a thin ribbon which is then focused into a single stream for analysis.
Claims9
A microfluidic device for analyzing particles dispersed in a sample fluid, comprising:
- a first inlet fluidically coupled to a first channel for introduction of the sample fluid;
- a second inlet fluidically coupled to a second channel for introduction of a first reagent fluid;
- a reactor channel fluidically coupled to the first channel and the second channel such that the sample fluid is focused into a thin ribbon between two streams of the first reagent fluid, the reactor channel having a length sufficient to allow a chemical reaction between the sample fluid and the first reagent fluid;
- a third inlet fluidically coupled to a third channel for introduction of a sheath fluid;
- a cytometer channel fluidically coupled to the reactor channel and the third channel such that the sample fluid is focused into a single file stream of particles between two streams of the sheath fluid;
- and
- a detection region.
4 more elements
A method for analyzing particles dispersed in a sample fluid, comprising:
- introducing the sample fluid and a first reagent fluid into a microfluidic device;
- focusing the sample fluid into a thin ribbon between two streams of the first reagent fluid;
- flowing the sample fluid and first reagent fluid through a reactor channel of the microfluidic device, the reactor channel having a length sufficient to allow a chemical reaction between the sample fluid and the first reagent fluid;
- introducing a sheath fluid into the microfluidic device;
- focusing the sample fluid into a single file stream of particles between two streams of the sheath fluid;
- and
- flowing the sample fluid, first reagent fluid and sheath fluid through a cytometer channel of the microfluidic device past a detection region of the microfluidic device.
4 more elements
Dependencies are inferred from the claim text.