Plant-optimized genes encoding pesticidal chimeric cry protein toxins
AssigneeMycogen Corporation
InventorsGuy A. Cardineau, Steven J. Stelman, Kenneth E. Narva
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Abstract
The subject invention provides a DNA construct comprising a promoter operably linked to a nucleic acid encoding an N-terminal core toxin and a C-terminal Cry1Ab protoxin portion, wherein the nucleic acid encoding the C-terminal Cry1Ab protoxin portion is a plant optimized polynucleotide sequence.
Claims10
A polynucleotide that encodes a chimeric Cry protein toxin and comprises a polynucleotide encoding an N-terminal core toxin portion operably linked to SEQ ID NO:9, which encodes a C-terminal protoxin portion.
A DNA construct that comprises a promoter active in a plant cell, wherein said promoter is operably linked to a polynucleotide that encodes a chimeric Cry protein toxin and comprises a polynucleotide encoding an N-terminal core toxin portion operably linked to SEQ ID NO:9, which encodes a C-terminal protoxin portion.
A method of producing transgenic plant cells, wherein said method comprises transforming a plant cell with a polynucleotide that encodes a chimeric Cry protein toxin, wherein said polynucleotide comprises a polynucleotide encoding an N-terminal core toxin portion operably linked to SEQ ID NO:9, which encodes a C-terminal protoxin portion;
- and culturing said plant cell under conditions whereby descendent generations of transgenic plant cells are produced.
Dependencies are inferred from the claim text.