Inhibitor of cell proliferation and methods of use thereof
AssigneeNucleus Remodeling, Inc.
InventorJoseph E. Zahner
Life of this patent
Estimating the term…
Abstract
An chimerical polypeptide that arrests proliferating cells in mitosis is provided. In general the polypeptide has an N-terminal transit peptide, such as HIV-1 Tat, and a C-terminal cell-cycle effector, such as a G2/M cyclin or a cytostatic factor. A polynucleotide under the control of a heterologous promoter that encodes a polypeptide that arrests cells in mitosis is also provided. The polynucleotide may, for example, encode a G2/M cyclin. Pharmaceutical compositions comprising the agent that inhibits transit through mitosis is provided. A method of treating patients suffering from a hyperplasia, such as cancer, psoriasis or benign prostate hyperplasia is provided.
Claims8
An isolated and purified recombinant polypeptide comprising a transit peptide domain and a cell-cycle effector domain, wherein said recombinant polypeptide can enter cells and inhibit cell cycle progression and the cell-cycle effector domain comprises a G2/M cyclin polypeptide.
A method of inhibiting proliferation of a cell in vitro comprising administering to the cell in vitro an isolated and purified recombinant polypeptide comprising a transit peptide domain and a cell-cycle effector domain, wherein said polypeptide enters the cell and inhibits cell cycle progression, and wherein the cell-cycle effector domain comprises a G2/M cyclin polypeptide.
Dependencies are inferred from the claim text.