Expression of exogenous polynucleotide sequences in a vertebrate, mammal, fish, bird or human

A method for delivering a biologically active protein, polypeptide or a peptide to a mammal comprising:

• directly administering to tissue of the mammal a construct comprising a non-infectious, non-integrating DNA sequence encoding the protein, polypeptide or peptide, wherein said DNA sequence is operably linked to a promoter in an amount sufficient that uptake of said construct by the tissue results in sufficient expression to produce the protein, polypeptide or peptide at a detectable level,
• wherein said DNA sequence is free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents.

A method for a delivering a biologically active protein, polypeptide or a peptide to a mammal comprising:

• injecting into muscle tissue of the mammal a construct comprising a non-infectious, non-integrating RNA sequence encoding the protein, polypeptide or peptide in an amount sufficient that uptake of said construct by the muscle tissue cells results in sufficient expression to produce the protein, polypeptide or peptide at a detectable level,
• wherein said RNA sequence is free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents.