Back to results
US 6,673,629 B2Granted
Neutralization of polycations in a chromatographic device for whole blood use
AssigneeAbbott Laboratories
InventorsToru Yoshimura, Toshihiro Ogasawara, Michihiro Saito, John P. Groff
Life of this patent
Estimating the term…
Abstract
A chromatography assay device and method for use with whole blood samples utilizing a red blood cell separating agent to aggregate red blood cells and permit plasma or serum to flow by capillary action and a neutralizing agent to neutralize any effects the red blood cell separating agent may have on the device and method.
Claims17
A chromatography assay device for detecting the presence of an analyte in a blood sample comprising:
- a chromatography carrier which defines a path for fluid flow and supports capillary flow,
- an application site for said blood sample in fluid flow contact with said chromatography carrier,
- a detection site on said chromatography carrier spaced apart from said application site having a non-diffusively bound trapping substance bound thereto,
- a diffusively bound labeled substance located downstream of said application site,
- a diffusively bound polycation for separating plasma or serum from said blood sample upstream of said detection site, and
- a diffusively bound polyanion for neutralizing the polycation downstream of said bound polycation and upstream of said detection site.
3 more elements
A method for detecting the presence of an analyte in a blood sample comprising the steps of:
- providing a chromatography carrier which defines a path for fluid flow and supports capillary flow, along which are (a) an application site for said blood sample in fluid flow contact with said chromatography carrier, (b) a detection site on said chromatography carrier spaced apart from said application site having a non-diffusively bound trapping substance bound thereto, (c) a labeled substance located downstream of said application site, (d) a diffusively bound polycation for separating plasma or serum from said blood sample upstream of said detection site, and (e) a diffusively bound polyanion for neutralizing the polycation located downstream of said bound polycation and upstream of said detection site;
- contacting said application site with said blood sample;
- and
- detecting the presence of an analyte in said blood sample.
1 more element
A method for detecting the presence of Human Immunodeficiency Virus type 1 or Human Immunodeficiency Virus type 2 in a blood sample comprising:
- providing a chromatography carrier which defines a path for fluid flow and supports capillary flow, along which are (a)an application site for said blood sample in fluid flow contact with said chromatography carrier,
- (b)a detection site on said chromatography carrier spaced apart from said application site having a non-diffusively bound trapping substance bound thereto,
- (c)a labeled substance located downstream of said application site,
- (d)a diffusively bound polycation for separating plasma or serum from said blood sample upstream of said detection site, and
- (e)a diffusively bound polyanion for neutralizing the polycation downstream of said bound polycation and upstream of said detection site;
- contacting said application site with said blood sample;
- and
- detecting the presence of the Human Immunodeficiency Virus type 1 or Human Immunodeficiency Virus type 2 in said blood sample.
5 more elements
A method for detecting the presence of Hepatitis B surface antigen in a blood sample comprising:
- providing a chromatography carrier which defines a path for fluid flow and supports capillary flow, along which are
- (a)an application site for said blood sample in fluid flow contact with said chromatography carrier,
- (b)a detection site on said chromatography carrier spaced apart from said application site having a non-diffusively bound trapping substance bound thereto,
- (c) a labeled substance located downstream of said application site,
- (d)a diffusively bound polycation for separating plasma or serum from said blood sample upstream of said detection site, and
- (e)a diffusively bound polyanion for neutralizing the polycation downstream of said bound polycation and upstream of said detection site;
- contacting said application site with said blood sample;
- and
- detecting the presence of the Hepatitis B Surface antigen in said blood sample.
6 more elements
A method for detecting the presence of Treponema pallidum in a blood sample comprising:
- providing a chromatography carrier which defines a path for fluid flow and supports capillary flow, along which are
- (a)an application site for said blood sample in fluid flow contact with said chromatography carrier,
- (b)a detection site on said chromatography carrier spaced apart from said application site having a non-diffusively bound trapping substance bound thereto,
- (c)a labeled substance located downstream of said site,
- (d)a diffusively bound polycation for separating plasma or serum from said blood sample upstream of said detection site, and
- (e)a diffusively bound polyanion for neutralizing the polycation downstream of said bound polycation and upstream of said detection site;
- contacting said application site with said blood sample;
- and
- detecting the presence of Treponema pallidum in said blood sample.
6 more elements
Dependencies are inferred from the claim text.