Use of CYP52A2A promoter to increase gene expression in yeast
AssigneeCognis Corporation
InventorsDavid L. Craft, C. Ron Wilson, Dudley Eirich, Yeyan Zhang
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Abstract
A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.
Claims67
An isolated nucleic acid molecule comprising a promoter of a Candida tropicalis CYP52A2A gene wherein the promoter consists of a sequence of about 495 nucleotides located upstream from the initiation codon of the CYP52A2A gene.
An isolated nucleic acid molecule comprising a promoter of a Candida tropicalis CYP52A2A gene wherein the promoter consists of a sequence of about 495 contiguous nucleotides as set forth in SEQ ID NO:10 and wherein the 495 contiguous nucleotides are located upstream from nucleotide 505 of SEQ ID NO:10.
An isolated nucleic acid molecule comprising a promoter of a Candida tropicalis CYP52A2A gene wherein the promoter consists of a sequence of about 495 contiguous nucleotides as set forth in SEQ ID NO:1 and wherein the 495 contiguous nucleotides are located upstream from nucleotide 1199 of SEQ ID NO:1.
An isolated nucleic acid molecule comprising a promoter of a yeast CYP52A2A gene wherein the promoter consists of nucleotides 9 to 504 of SEQ ID NO:10.
An isolated nucleic acid molecule comprising a promoter of a yeast CYP52A2A gene wherein the promoter consists of nucleotides 703 to 1198 of SEQ ID NO:1.
Dependencies are inferred from the claim text.