Somatic embryogenic regeneration of Acacia mangium
AssigneeInstitute of Molecular Agrobiology
InventorsXie Deyu, Hong Yan
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Abstract
The present invention relates to the field of somatic embryo production, particularly to methods for the regeneration of Acacia mangium through somatic embryogenesis. More specifically, the present invention relates to a method for regeneration of plants of Acacia mangium by culturing explants of immature zygotic embryos on callus induction medium to grow embryogenic tissue. Culturing of the embryogenic tissue is continued on somatic embryo maturation medium and germination medium. The germinated embryos are further converted to acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation.
Claims21
A method for preparing somatic embryos of Acacia mangium comprising:
- a) culturing an explant on callus induction medium to induce embryogenic callus and globular embryo formation;
- and
- b) culturing globular embryos on a maturation medium to form somatic embryos.
A method for regenerating Acacia mangium comprising:
- a) culturing an explant on callus induction medium to induce embryogenic callus and globular embryo formation;
- b) culturing the globular embryos on a maturation medium to form somatic embryos;
- and
- c) culturing the somatic embryos on a germination medium to produce regenerated seedlings.
Dependencies are inferred from the claim text.