Disposable culture bag
AssigneeSynthecon, Inc.
InventorsRoger Akers, William J. Anderson, Stephen S. Navran, Jr., Adrian F. Dinges, Jr.
Life of this patent
Estimating the term…
Abstract
A cell culture bag for culturing cells, cellular aggregates, particles, tissues and organoids is disclosed. The cell culture bag is a flexible and disposable culture bag that fits within a bag support chamber of a bioreactor. The cell culture bag has an inlet means and an outlet means for the introduction and removal of media. Certain embodiments of the culture bag have an inlet and outlet fluid coupling swivel joint and/or perforated perfusion tubes extending from the inlet means into the interior of the bag to assist in mass transfer and mixing the media to maintain an even temperature throughout the culture bag.
Claims51
A culture bag comprising:
- at least one sheet having a first end, a second end, an internal side, and an external side, wherein the internal side of said sheet is positioned to face an interior of the culture bag;
- a seam formed by fusing overlapped sections of one or more sheets;
- an inlet means fused to the first end of one or more sheet;
- an outlet means fused to the second end of one or more sheet;
- and
- at least one outer perfusion tube, wherein a proximal end of the outer perfusion tube is in fluid communication with the inlet means, a central section of an external surface of the outer perfusion tube is fused to an interior-surface of the seam, and a distal end of the outer perfusion tube is closed.
A culture bag comprising:
- a plurality of sheets having a first end, a second end, an internal side, an external side and two opposed edges, wherein one edge of each sheet is fused with one edge of an adjacent sheet to form a seam, and wherein the internal sides of said fused sheets are positioned within an interior of the bag;
- an inlet means fused to the first end of at least one sheet, wherein the inlet means includes an inlet end piece and an inlet fluid coupling swivel joint;
- and
- an outlet means fused to the second end of at least one sheet, wherein the outlet means comprises an outlet end pierce and an outlet fluid coupling swivel joint.
A culture bag comprising:
- at least one sheet having a first end, a second end, an internal side, and an external side, wherein the internal side of said sheet is positioned to face an interior of the culture bag;
- a seam formed by fusing overlapped sections of one or more sheets;
- an inlet means fused to the first end of one or more sheet;
- an outlet means fused to the second end of one or more sheet;
- and
- at least one outer perfusion tube having a proximal end, a central section and a distal end, wherein the proximal end is in fluid communication with the inlet means, a portion of an external surface of the central section is fused to an interior surface of the seam, and the distal end is closed, and further wherein the central section of the outer perfusion tube is perforated to allow fluid communication between a lumen of the outer perfusion tube and the interior of the culture bag;
- and
- at least one perforated internal perfusion tube, said internal perfusion tube in fluid communication with the inlet means and the interior of the culture bag.
A culture bag comprising:
- at least one sheet having a first end, a second end, an internal side, and an external side, wherein the internal side of said sheet is positioned to face an interior of the culture bag;
- a seam formed by fusing overlapped sections of one or more sheets;
- an inlet means fused to the first end of one or more sheet, wherein the inlet means includes an inlet end piece, an inlet fluid coupling swivel joint, a manifold and a branch port;
- an outlet means fused to the second end of one or more sheet, wherein the outlet means includes an outlet end piece and an outlet fluid coupling swivel joint;
- and
- a perforated perfusion tube, an external surface of a proximal end of the perfusion tube fused to the branch port and a lumen of the perfusion tube in communication with the manifold and the interior of the culture bag.
Dependencies are inferred from the claim text.