α-amylase mutants
AssigneeNovozymes A/S
InventorsTorben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Søren Kjærulff
Life of this patent
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Abstract
The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
Claims34
A variant of a parent Termamyl-like α-amylase, wherein said variant has α-amylase activity and at least 80% sequence identity to said parent α-amylase and comprises one or more mutations at a position corresponding to a position in the amino acid sequence shown in SEQ ID NO:
- 2 selected from the group consisting of:
- T461P;
- Q174*;
- R181Q,N,S;
- and
- G182T,S,N.
Dependencies are inferred from the claim text.