Method for cloning and expression of MspA1l restriction endonuclease and MspA1l methylase in E. coli
AssigneeNew England Biolabs, Inc.
InventorsShuang-yong Xu, Robert Maunus, Katy Stropnicky
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Abstract
The present invention relates to recombinant DNA coding for the MspA1I restriction endonuclease as well as MspA1I methylase, expression of MspA1I restriction endonuclease and MspA1I methylase in E. coli cells containing the recombinant DNA.
Claims6
Isolated DNA encoding the MspA1I restriction endonuclease, wherein the isolated DNA is obtainable from ATCC No. PTA-4046.
A recombinant DNA vector comprising a vector into which a DNA segment encoding the MspA1I restriction endonuclease has been inserted.
Isolated DNA encoding the MspA1I restriction endonuclease and MspA1I methylase, wherein the isolated DNA is obtainable from ATCC No. PTA-4046.
Dependencies are inferred from the claim text.