Loading patent…
AssigneeTakara Shuzo Co., Ltd.
InventorsTakashi Uemori, Yoshimi Sato, Mariko Okawa, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroaki Sagawa, Michio Hagiya, Hiroyuki Mukai, Kiyozo Asada, Ikunoshin Kato
Estimating the term…
A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 μl of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 μl volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99° C., 1 second-66° C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.
A DNA synthesis method with a shortened time period for DNA synthesis by polymerase chain reaction (PCR), comprising the steps of:
A method for determining an effective amount of DNA polymerase useful for performing rapid PCR comprising the steps of:
Dependencies are inferred from the claim text.