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AssigneeCuraGen Corporation
InventorsJonathan M. Rothberg, Michael W. Deem, John W. Simpson
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The present invention discloses a methodology which is directed to providing positive confirmation that nucleic acids, possessing putatively identified sequences predicted to generate observed GeneCalling™ signals, are actually present within the sample from which the signal was originally derived. The putatively identified nucleic acid fragment within the sample possesses 3′- and 5′-ends with known terminal subsequences. The method involves contacting nucleic acid fragments in a sample in amplifying conditions with (i) a nucleic acid polymerase; (ii) “regular” primer oligonucleotides having sequences comprising hybridizable portions of known terminal subsequences; and (iii) a “poisoning” oligonucleotide primer. Nucleic acids amplified with a poisoning primer are distinguishable upon detection from nucleic acids amplified with regular primers.
A method for identifying one or more nucleic acids in a sample comprising a plurality of nucleic acid fragments having different nucleotide sequences, wherein said nucleic acid fragments within said sample possess known 3′- and 5′-terminal subsequences, said method comprising:
A method of confirming a putatively identified sequence of a nucleic acid fragment in a sample of nucleic acid fragments, wherein each nucleic acid fragment in said sample has ends with known terminal subsequences, said method comprising:
Dependencies are inferred from the claim text.