Process for producing nucleic acids
AssigneeAjinomoto Co., Inc.
InventorsYoshihiro Usuda, Hisashi Kawasaki, Megumi Shimaoka, Takashi Utagawa
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Abstract
The present invention relates to a process for producing 5′-inosinic acid or 5′-guanylic acid for use in seasonings or the like from inosine or guanosine or a precursor thereof using an adenosine triphosphate (ATP)-regenerating microorganism containing a DNA encoding a protein that has the activity of forming 5′-inosinic acid or 5′-guanylic acid from inosine or guanosine. Further, the present invention relates to a novel protein having the inosine-guanosine kinase activity, a gene encoding said protein, a recombinant DNA containing said gene, and a microorganism which is transformed with said recombinant DNA.
Claims7
A process for producing 5′-inosinic acid or 5′-guanylic acid, which comprises contacting inosine or guanosine or a precursor thereof with a transformant obtained by introducing a gene encoding a protein comprising SEQ ID NO:
- 3 or 17 having inosine-guanosine kinase activity into microorganism capable of regenerating ATP consumed in the process, an energy source and a phosphate group donor, accumulating 5′-inosinic acid or 5′-guanylic acid in the reaction solution, and collecting the same therefrom, where the phosphate group donor is an inorganic phosphoric acid, a salt of an inorganic phosphoric acid, phenyl phosphate, acetyl phosphate or carbamyl phosphate.
A process for producing 5′-inosinic acid or 5′-guanylic acid, which comprises contacting inosine or guanosine or a precursor thereof with a transformant obtained by introducing (a) a gene encoding a protein having inosine-guanosine kinase activity into (b) a microorganism capable of regenerating ATP consumed in the process, an energy source and a phosphate group donor, accumulating 5′-inosinic acid or 5′-guanylic acid in the reaction solution, and collecting the same therefrom, where the phosphate group donor is an inorganic phosphoric acid, a salt of an inorganic phosphoric acid, phenyl phosphate, acetyl phosphate or carbamyl phosphate, wherein the gene (a) is a gene having the sequence of SEQ ID NO:1, wherein said transformant is the only microorganism employed in the stated process.
Dependencies are inferred from the claim text.