Method for preparing polypeptides with appropriate glycosilation
AssigneeRoche Diagnostics GmbH
InventorsReinhard Franze, Horst Eberhardt, Claus Wallerius
Life of this patent
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Abstract
The invention concerns a process for the production of a polypeptide with suitable glycosylation by culturing eukaryotic cells and isolating the polypeptide from the culture medium or/and the cells. In this process the desired glycosylated polypeptide can be produced recombinantly with the aid of endogenous gene activation or be produced naturally by the cells.
Claims11
A process for increasing the glycosylation of a polypeptide when producing glycosylated polypeptides in mammalian or insect cells while maintaining cell specific productivity comprising:
- (a) culturing mammalian or insect cells in a culture medium;
- (b) monitoring the concentration of at least one parameter selected from nutrients and metabolites of the cultured cells wherein said parameter correlates to the nutrient requirement of the cells and determining the consumption rate of said parameter;
- (c) adding a mixture of at least two carbohydrates to the culture medium in an amount sufficient to meet the nutrient requirements of the cells to maintain cell specific productivity, wherein said amount is based on the consumption rate of the parameter in step (b);
- and
- (d) isolating the glycosylated polypeptide from the culture medium,
- wherein culturing the cells under the condition (a)-(c) increases the glycosylation of the polypeptide while maintaining cell specific productivity.
A process for increasing the glycosylation of a polypeptide when producing glycosylated polypeptides in mammalian or insect cells while maintaining cell specific productivity comprising:
- (a) culturing mammalian or insect cells in a culture medium;
- (b) monitoring the concentration of at least one parameter selected from nutrients and metabolites wherein said parameter correlates with the nutrient requirements of the cells and determining the consumption rate of said parameter;
- (c) adding a nutrient mixture comprising at least one essential amino acid and/or at least one carbohydrate to the medium in an appropriate amount sufficient to meet the nutrient requirements of the cells, wherein such amount is based on the consumption rate of the parameter in step (b);
- and
- (d) isolating the glycosylated polypeptide from the culture medium,
- wherein culturing the cells under the conditions (a)-(c) increases the glycosylation of the potypeptide while maintaining cell specific productivity.
Dependencies are inferred from the claim text.