Smad associating polypeptides
AssigneeLudwig Institute for Cancer Research
InventorsFumiko Itoh, Susumu Itoh, Carl-Henrik Heldin, Peter ten Dijke
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Abstract
The invention describes Smad associating proteins (SAPs) and nucleic acids that encode SAPs, including fragments and biologically functional variants thereof, as well as antibodies that bind thereto. Methods and products for using such nucleic acids and polypeptides also are provided.
Claims20
An isolated nucleic acid molecule selected from the group consisting of
- (a) nucleic acid molecules which hybridize under stringent conditions to a molecule consisting of the nucleic acid sequence set forth in SEQ ID NO:3 and which code for a polypeptide which binds Smad6, wherein the stringent conditions are hybridization at 65° C. in hybridization buffer (3.5×SSC, 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% Bovine Serum Albumin, 2.5 mM NaH2PO4(pH7), 0.5% SDS 2 mM EDTA). SSC, is 0.15M sodium chloride/0.015M sodium citrate, pH7;
- SDS is sodium dodecyl sulphate;
- and EDTA is ethylenediaminetetracetic acid,
- (b) nucleic acid molecules that differ from the nucleic acid molecules of (a) in codon sequence due to the degeneracy of the genetic code, and
- (c) complements of (a) and (b).
An isolated nucleic acid molecule selected from the group consisting of (a) a fragment of nucleotides 1-2399 of SEQ ID NO:3 between 12 and 2398 nucleotides in length that codes for a polypeptide that binds Smad6, and (b) complements of “(a)”, provided that the nucleic acid molecule excludes sequences consisting of SEQ ID NO:17, SEQ ID NO:18 and SEQ ID NO:19.
Dependencies are inferred from the claim text.