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US 6,673,551 B2Granted
Probes for variance detection
AssigneeVariagenics, Inc.
InventorVincent P. Stanton, Jr.
Life of this patent
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Abstract
Disclosed are methods and reagents for detecting nucleotide mismatches (for example, due to sequence variances) in a nucleic acid sample involving the use of a nucleic acid probe derived from a hemizygous cell. Methods for determining the haplotype of a nucleic acid sample are also disclosed. Also disclosed are methods for producing the probe and kits containing the probe.
Claims6
A method for analyzing a test nucleic acid sample to determine whether it contains a sequence variance, said method comprising the steps of:
- (a) obtaining a nucleic acid probe that is derived from a hemizygous cell and that is complementary to a sex chromosome or segment thereof;
- (b) forming a duplex between said test nucleic acid sample and said probe;
- and
- (c) analyzing whether said duplex contains a nucleotide mismatch, thereby determining whether said test nucleic acid sample contains a sequence variance.
1 more element
Dependencies are inferred from the claim text.