Tuberculosis vaccine
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsStefan H. E. Kaufmann, Jürgen Hess
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Abstract
The present invention relates to novel recombinant vaccines providing protective immunity against tuberculosis. Further, the present invention refers to novel recombinant nucleic acid molecules, vectors containing said nucleic acid molecules, cells transformed with said nucleic acid molecules and polypeptides encoded by said nucleic acid molecules.
Claims33
A recombinant nucleic acid molecule encoding a fusion polypeptide, said fusion polypeptide comprising
- (a) at least one domain from a Mycobacterium polypeptide, wherein said domain is for eliciting an immune response in a mammal, and
- (b) a Listeria phagolysosomal escape domain.
A recombinant Mycobacterium bovis cell, comprising at least one recombinant nucleic acid molecule encoding a fusion polypeptide, said fusion polypeptide comprising
- (a) at least one domain from a Mycobacterium polypeptide and for eliciting an immune response in a mammal and
- (b) a Listeria phagolysosomal escape domain.
A recombinant Mycobacterium bovis cell comprising at least one recombinant nucleic acid molecule encoding a Listeria phagolysosomal escape peptide or polypeptide.
An immunogenic polypeptide comprising (a) at least one domain from a Mycobacterium polypeptide, wherein said domain elicits an immune response in a mammal, and (b) a Listeria phagolysosomal escape domain.
A recombinant nucleic acid molecule encoding a fusion polypeptide, said fusion polypeptide comprising
- (a) at least one domain from a Mycobacterium polypeptide, wherein said domain is for eliciting an immune response in a mammal, and
- (b) a Listeria phagolysosomal escape domain encoded by a nucleic acid molecule selected from the group consisting of:
- (i) the nucleotide sequence from nucleotide 211-1722 as shown in SEQ ID NO:
- 1,
- (ii) a degenerate nucleotide sequence encoding an amino acid sequence corresponding to the nucleotide sequence of (a), and
- (iii) a nucleotide sequence hybridizing under stringent conditions with the nucleotide sequence of (a) or (b).
A recombinant nucleic acid molecule encoding a fusion polypeptide, said fusion polypeptide comprising
- (a) at least one domain from a Mycobacterium polypeptide, wherein said domain is for eliciting an immune response in a mammal, and
- (b) a phagolysosomal escape domain encoded by a nucleic acid molecule selected from the group consisting of:
- (i) the nucleotide sequence from nucleotide 211-1722 as shown in SEQ ID NO:
- 1.
A recombinant nucleic acid molecule encoding a fusion polypeptide, said fusion polypeptide comprising
- (a) at least one domain from a Mycobacterium polypeptide, wherein said domain is for eliciting an immune response in a mammal and said domain is selected from the group consisting of Mycobacterium antigens Ag85B (M. tuberoulosis), Ag85B (M. bovis), Ag85A (M.tuberculosis) and ESAT-6 (M. tuberculosis), or an immunogenic fragment thereof, and
- (b) a Listeria phagolysosomal escape domain.
Dependencies are inferred from the claim text.