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US 6,670,186 B1Granted
RNA-loaded antigen presenting cells
AssigneeDuke University
InventorsSmita K. Nair, David J. Boczkowski, Eli Gilboa
Life of this patent
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Abstract
The present invention relates, in general, to RNA-loaded antigen presenting cells and, in particular, to methods for treating or preventing tumor formation or pathogen infection in a patient. The invention further relates to methods of monitoring T-cell stimulation and to methods of antigen discovery.
Claims14
A method for detecting a T cell response against a tumor antigen comprising:
- i) isolating RNA from tumor cells,
- ii) introducing said RNA resulting from step (i) into antigen presenting cells (APCs) isolated from an individual to produce target cells that functionally present on the surface thereof an antigen encoded in said RNA,
- iii) contacting said target cells with T-cells from said individual, and
- iv) assaying:
- a) for cytotoxicity wherein lysis of said target cells indicates that said individual has T cells that recognize said tumor antigen, or
- b) for an increase in cytokine production by said T cells, relative to a level of cytokine production in T cells not contacted with said target cells, wherein an increase in cytokine production indicates that said individual has T cells that recognize said tumor antigen.
3 more elements
A method for detecting a T cell response against a pathogen antigen comprising:
- i) isolating RNA from a pathogen,
- ii) introducing said RNA resulting from step (i) into antigen presenting cells (APCs) isolated from an individual to produce target cells that functionally present on the surface thereof an antigen encoded in said RNA,
- iii) contacting said target cells with T-cells from said individual, and
- iv) assaying:
- a) for cytotoxicity wherein lysis of said target cells indicates said individual has T cells that recognize said pathogen antigen, or
- b) for an increase in cytokine production by said T cells, relative to a level of cytokine production in T cells not contacted with said target cells, wherein an increase in cytokine production indicates that said individual has T cells that recognize said pathogen antigen.
3 more elements
Dependencies are inferred from the claim text.