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AssigneeAthersys, Inc.
InventorsJohn J. Harrington, Bruce Sherf, Stephen Rundlett
Estimating the term…
The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention also provides methods for isolation of nucleic acid molecules (particularly cDNA molecules) encoding a variety of proteins. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.
A vector comprising, in sequential order:
A vector comprising, in sequential order:
An animal cell in vitro wherein at vector is integrated into the genome of said animal cell, said vector comprising, in sequential order:
A method for increasing expression of an endogenous gene in an animal cell in vitro, said method comprising introducing a vector into said cell, said vector comprising, in sequential order:
A method for identifying a desired endogenous gene in an animal cell in vitro, said method comprising introducing a vector into said animal cell, said vector comprising, in sequential order:
A method for identifying an endogenous gene, said method comprising introducing a vector into an animal cell in vitro, said vector comprising, in sequential order:
A method for cleaving a cDNA molecule with a restriction enzyme, said cDNA molecule being derived from an unspliced cellular transcript, said method comprising:
A vector comprising, in sequential order:
A vector comprising, in sequential order:
A vector comprising, in sequential order:
An animal cell in vitro expressing an endogenous gene, said cell comprising a vector that comprises in sequential order:
Dependencies are inferred from the claim text.