Method for producing a lipid emulsion for use in insect cell culture
AssigneeBASF SE
InventorsRonald H. Chiarello, Sarah Himmerich
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Abstract
A serum-free insect cell culture medium is provided which provides improvements in the maximum insect cell density and replication of insect viruses within these cells, at a significantly lower cost than commercially-available media. A method is provided for preparing a lipid emulsion for use in insect culture media for large scale culture, and which produces a more stable emulsion with a longer shelf life. The method is preferably carried out by combining in an organic solvent a surfactant and a mixture of lipids to make a lipid phase, heating the lipid phase to a temperature of 40 to 70 degrees Celsius to form an anhydrous lipid phase and adding an aqueous phase thereto to provide a stable lipid micro-emulsion.
Claims5
A method for producing a lipid emulsion at large scale for use in cell culture media, said method comprising:
- combining in an organic solvent a surfactant and a mixture of lipids to create a lipid phase, wherein said lipids are selected from the group consisting of fatty acids, sterols, and lipid-soluble vitamins;
- heating said lipid phase to a temperature of 40° C. to 70° C. to create an anhydrous lipid phase;
- and
- adding an aqueous phase to said anhydrous lipid phase to produce a stable lipid micro-emulsion, wherein said aqueous phase consists of water.
Dependencies are inferred from the claim text.