Assays for β-amyloid processing
AssigneesAventis Pharmaceuticals Inc., Scios Nova Inc.
InventorsRebecca Pruss, John Huggins, Guy Rautmann, Barbara Cordell, Jan Marian Scardina, Ronald P. Mischak
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Abstract
The invention relates to the design, construction and use of eukaryotic cell lines useful in the identification of inhibitors of β-amyloid processing. More specifically, the invention relates to in vitro assays capable of identifying or quantifying a 4.2 kDa β-amyloid protein. The present invention also provides for DNA and protein molecules for the design, construction and use of eukaryotic cell lines useful in the identification of inhibitors of β-amyloid processing.
Claims26
An eukaryotic cell line having a exogenous gene construction, said exogenous gene construction comprising a cytomegalovirus promoter encoding sequence, a strong ribosome binding site encoding sequence, a β-amyloid precursor protein encoding sequence, a selectable marker encoding sequence, and a poly-adenylation signal encoding sequence.
A eukaryotic cell line selected from the group consisting of 21-N-1, 21-N-2, 21-N-3, 21-N-4, 21-N-5, 21-N-6, 21-N-7, 21-N-8, and 21-N-9.
An eukaryotic cell line having a exogenous gene construction, said exogenous gene construction comprising a cytomegalovirus promoter encoding sequence, a ribosome binding site encoding sequence, a β-amyloid precursor protein encoding sequence, a selectable marker encoding sequence, and a poly-adenylation signal encoding sequence.
A substantially purified nucleic acid molecule that encodes, in order:
- a cytomegalovirus promoter;
- a ribosome binding site;
- a β-amyloid precursor protein molecule;
- a selectable marker;
- and
- a poly-adenylation signal.
A substantially purified nucleic acid molecule that encodes, in order:
- a cytomegalovirus promoter;
- a strong ribosome binding site;
- a β-amyloid precursor protein molecule;
- a selectable marker;
- and
- a poly-adenylation signal.
A nucleic acid molecule comprising pCMV-IRES-βAPP695.
A composition comprising a eukaryotic cell line and an inhibitor of a β-amyloid processing pathway, said eukaryotic cell line having a exogenous gene construction, said exogenous gene construction comprising a cytomegalovirus promoter encoding sequence, a strong ribosome binding site encoding sequence, a β-amyloid precursor protein encoding sequence, a selectable marker encoding sequence, and a poly-adenylation signal encoding sequence.
Dependencies are inferred from the claim text.