Compositions and methods for treating viral infections
AssigneeVirionyx Corporation
InventorFrank B. Gelder
Life of this patent
Estimating the term…
Abstract
Methods and compositions for treatment, diagnosis, and prevention of a virus comprise administering to a patient antibodies which react with regions of viral proteins and result in neutralization of infectivity and inactivation of functionally essential events in the life cycle of the virus. The antibodies recognize viral epitopes which fail to elicit an immune response in man when encountered through infection or naturally through the environment. In a preferred embodiment, the invention provides compositions and methods useful in the treatment and diagnosis of human immunodeficiency virus (HIV) infections.
Claims39
A method for treating a patient infected with HIV, so as to ameliorate the patient's clinical condition, the method comprising:
- administering to said patient a therapeutically effective amount of a composition comprising one or more antibodies each of which recognizes and reacts with an epitope, which epitope corresponds to or mimics an epitope on a neutralizing or inactivating region of an HIV protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered by infection or environmental exposure, but does elicit an immune response in a non-human mammal.
A method for neutralizing or inactivating one or more essential steps in the life cycle of HIV in a patient infected with the virus which comprises administering to said patient a therapeutically effective amount of a composition comprising one or more antibodies each of which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of an HIV protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered by infection or environmental exposure, but does elicit an immune response in a non-human mammal.
A method for treating a patient infected with HIV, so as to achieve one or more clinical goals selected from the group consisting of:
- a) an increase in the number of the patient's CD4 cells per unit volume of blood,
- b) an increase in the number of the patient's CD80 cells per unit volume of blood, and
- c) an decrease the viral load of the patient the method comprising:
- administering to said patient a therapeutically effective amount of a composition comprising one or more antibodies each of which recognizes and reacts with an epitope, which epitope corresponds to or mimics an epitope on a neutralizing or inactivating region of an HIV protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered by infection or environmental exposure, but does elicit an immune response in a non-human mammal.
A method for detecting the presence of HIV in a biological fluid sample from an individual who may have been infected with HIV which comprises employing an antibody which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of an HIV protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered through infection or environmental exposure in an antibody-antigen assay in which said antibody is combined with a sample of body fluid from said individual under conditions conducive to antibody-antigen complex formation and determining whether said antibody binds to an HIV antigen.
A method for detecting the presence of HIV in an individual who may have been infected with HIV which comprises employing an antibody which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of an HIV protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered through infection or environmental exposure in an enzyme immunoassay, wherein said antibody is conjugated to an enzyme and contacted with a sample of body fluid from said individual under conditions conducive to antibody-antigen complex formation, and determining whether said antibody binds to an HIV antigen.
A method for purifying protein containing at least one epitope which corresponds to or mimics an epitope of a neutralizing or inactivating region of an HIV protein from a protein solution, which comprises immobilizing an antibody which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of an HIV protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered through infection or environmental exposure to a substrate or solid support, contacting said immobilized antibody with a solution containing said protein under conditions suitable for the formation of immune complexes between said antibody and said protein, separating unbound protein from protein bound to said antibody, and releasing said bound protein from said antibody and recovering said protein.
A method for treating a patient infected with a virus, the method comprising:
- administering to said patient a therapeutically effective amount of a composition comprising one or more antibodies each of which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of a protein of the virus, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered through infection or environmental exposure, but does elicit an immune response in a non-human mammal.
A method for detecting the presence of a virus in a biological fluid sample from an individual who may have been infected with the virus which comprises employing an antibody which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of a viral protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered through infection or environmental exposure in an antibody-antigen assay in which said antibody is combined with a sample of body fluid from said individual under conditions conducive to antibody-antigen complex formation and determining whether said antibody binds to an antigen of the virus.
A method for detecting the presence of a virus in an individual who may have been infected with the virus which comprises employing an antibody which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of a viral protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered through infection or environmental exposure in an enzyme immunoassay, wherein said antibody is conjugated to an enzyme and contacted with a sample of body fluid from said individual under conditions conducive to antibody-antigen complex formation and determining whether said antibody binds to an antigen of the virus.
A method for purifying protein containing at least one epitope which corresponds to or mimics an epitope of a neutralizing or inactivating region of a viral protein from a protein solution, which comprises immobilizing an antibody which recognizes and reacts with an epitope which corresponds to or mimics an epitope on a neutralizing or inactivating region of a viral protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered through infection or environmental exposure to a substrate or solid support, contacting said immobilized antibody with a solution containing said protein under conditions suitable for the formation of immune complexes between said antibody and said protein, separating unbound protein from protein bound to said antibody, and releasing said bound protein from said antibody and recovering said protein.
Dependencies are inferred from the claim text.