Disposable vessel
AssigneeWheaton USA, Inc.
InventorKenneth B. Carll
Life of this patent
Estimating the term…
Abstract
The present invention relates to an improved vessel for cell culture and methods for its use. The vessel of the present invention comprises a collapsible bag with an inner surface, an outer surface and a top periphery and a headplate having a circumferential edge wherein the top periphery of the bag is sealed to the edge of the headplate. The present invention also relates to an impeller for use with the vessel and a method for culturing cells.
Claims27
A vessel for cell culture comprising:
- a headplate having a circumferential edge;
- a collapsible bag with an inner surface, an outer surface and a top periphery, said top periphery of said bag sealed to said edge of said headplate;
- and
- an impeller comprised of a flexible blade and a hollow flexible shaft, said shaft having a top region, a bottom region, and a central axis, said impeller attached to said headplate at said shaft's top region such that said impeller does not rotate about its central axis.
A vessel for cell culture comprising:
- a headplate;
- a pre-sterilized collapsible bag sealed to said headplate;
- an impeller comprising a hollow flexible shaft connected to said headplate;
- two flexible blades attached to said impeller;
- and
- a constriction device o-ring disposed on said flexible shaft.
An impeller comprising a hollow flexible shaft containing a magnet and having a top region and a bottom region, said bottom region having a flexible blade, said magnet is removable through the top of said hollow flexible shaft.
A method of mixing a fluid comprising the steps of:
- providing a vessel comprising a collapsible bag containing an impeller comprised of a hollow flexible shaft;
- inserting a magnet into said hollow shaft of said impeller;
- introducing an external magnetic source to interact with said magnet and cause said magnet and said hollow shaft to move;
- and
- removing said magnet through the top of said hollow shaft of said impeller.
The method of culturing cells in a pre-sterilized vessel comprising a collapsible bag with a headplate and an impeller comprised of a hollow flexible shaft having a top region and a bottom region, wherein said top region is connected to said headplate and wherein said bottom region comprises a flexible blade comprising the steps of the method of:
- inserting a magnet into said hollow shaft of said impeller;
- introducing a cell line and media into said vessel;
- allowing said cell line to proliferate;
- removing said cell line and media from said vessel;
- removing said magnet through the top of said hollow shaft of said impeller;
- and
- disposing of said vessel.
A method of culturing cells in a collapsible vessel containing an impeller having a hollow shaft, the method comprising the steps of:
- inserting a magnet into said hollow shaft;
- introducing an external magnetic source to interact with said magnet and cause said magnet and said hollow shaft to move;
- and
- removing said magnet through the top of said hollow shaft.
A vessel comprising:
- a headplate having a circumferential edge;
- a collapsible bag with an inner surface, an outer surface, and a top periphery, with said top periphery of said bag sealed to said edge of said headplate;
- and
- an impeller comprised of a flexible blade and a hollow flexible shaft, said shaft having a top region, a bottom region, and a central axis, said impeller attached to said headplate at said shaft's top region such that said impeller does not rotate about its central axis.
A vessel comprising:
- a headplate;
- a pre-sterilized collapsible bag sealed to said headplate;
- an impeller comprising a hollow flexible shaft connected to said headplate;
- two flexible blades attached to said impeller;
- an o-ring disposed on said flexible shaft;
- and
- a port disposed within said headplate for accessing said hollow flexible shaft of said impeller.
A method of mixing the contents of a vessel containing an impeller having a hollow shaft, the method comprising the steps of:
- inserting a magnet into the hollow shaft through the top of the hollow shaft;
- and
- introducing an external magnetic source to interact with the magnet and cause the magnet and the hollow shaft to move.
A method of mixing the contents of a pre-sterilized vessel comprising a collapsible bag with a headplate and an impeller comprised of a hollow flexible shaft having an interior, a top region, and a bottom region, wherein said top region is connected to said headplate such that the interior of said shaft communicates with a hole through said headplate and wherein said bottom region comprises a flexible blade comprising the steps of:
- inserting a magnet into the hollow shaft of the impeller through the hole in the headplate;
- introducing contents into said vessel;
- introducing an external magnetic source to interact with the magnet and cause the magnet and the hollow shaft to move;
- allowing said contents to mix;
- removing said contents from said vessel;
- and
- disposing of said vessel.
Dependencies are inferred from the claim text.