Temperature-regulated cell perifusion chamber

A cell perifusion chamber structure comprising:

• a cell chamber body having a support surface with an aperture defined through said support surface, and wall structure extending upwardly from said support surface to define an interior, said wall structure including passages therein,
• a gasket disposed on said support surface so as not to cover said aperture,
• a first transparent cover disposed on said gasket so as to cover said aperture,
• a water bath body having a first portion and a second portion extending from said first portion, said first portion defining a second support surface, said second portion being received in said interior of said cell chamber body and being in interference fit arrangement with said wall structure, said water bath body having an interior support surface with an aperture therethrough, said aperture extending through said first and second portions, said first portion having first ports therein which communicate with said aperture of said water bath body, said second portion including second ports therein which communicate with said aperture of said water bath body and with associated said passages in said cell chamber body,
• a second transparent cover disposed on said interior support surface of said water bath body so as to divide said aperture of said water bath body into first and second portions, said second transparent cover covering said second portion of said aperture to define a sealed cell chamber enclosed by said second transparent cover, said first transparent cover and surfaces of said cell chamber body,
• a transparent window disposed on said second support surface to cover said first portion of said aperture to define a water bath chamber enclosed by said transparent window, said second cover and surfaces of said water bath body, whereby the chamber is constructed and arranged to permit fluid to enter and exit said water bath chamber via said first ports to regulate temperature in said cell chamber via heat exchange therewith, and to permit perifusion fluid to enter and exit said cell chamber via said second ports,
• wherein said first and second portions of said water bath body are each cylindrical and said second portion has a diameter less than said first portion, said wall structure defining a cylindrical interior for receiving said second portion.