Compositions and methods for template-dependent enzymatic synthesis of nucleic acid
AssigneeThe Board of Trustees of the Leland Stanford Jr. University
InventorsWilliam C. Copeland, Teresa S. F. Wang
Life of this patent
Estimating the term…
Abstract
The human DNA polymerase α catalytic polypeptide has been functionally over-expressed by a recombinant baculovirus in insect cells at >1000 fold higher levels than that found in cultured normal human cells.
Claims9
A composition comprising a full-length human DNA polymerase-α catalytic polypeptide subunit having the amino acid sequence of SEQ ID NO:31, wherein said subunit has polymerase activity and wherein said composition lacks primase activity.
A purified preparation, comprising human DNA polymerase-α catalytic polypeptide having the amino acid sequence of SEQ ID NO:31, wherein said preparation has a specific activity of approximately 200,000 units/mg and lacks primase activity.
A composition comprising a full-length enzymatically active human DNA polymerase-α catalytic polypeptide subunit having the amino acid sequence of SEQ ID NO:31, wherein said subunit has polymerase activity, wherein said composition lacks primase activity, and further wherein said composition has a specific activity of approximately 200,000 units/mg.
Dependencies are inferred from the claim text.