α1,2-fucosyltransferase
AssigneeThe Governors of the University of Alberta
InventorsDiane Taylor, Ge Wang, Monica Palcic
Life of this patent
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Abstract
A bacterial α1,2-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing α1,2-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, Lewis B and H type 1, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors.
Claims15
An isolated polynucleotide comprising a fragment of SEQ ID NO:
- 1 that encodes a α1,2-fucosyltransferase polypeptide that catalyzes the synthesis of Lewis Y and Lewis B.
An isolated polynucleotide that encodes a α1,2-fucosyltransferase polypeptide comprising SEQ ID NO:
- 2 or a bioactive fragment thereof, wherein the polypeptide or bioactive fragment has α1,2-fucosyltransferase activity and catalyzes the synthesis of Lewis Y and Lewis B.
An isolated polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising SEQ ID NO:
- 1;
- (b) a polynucleotide comprising nucleotide 137-1036 of SEQ ID NO:
- 1, wherein “T” in the sequence is replaced with “U”;
- (c) a polynucleotide that hybridizes to a nucleic acid molecule comprising a sequence as set forth in SEQ ID NO:
- 1 under stringent conditions wherein said stringent conditions comprise hybridization at 68° C. in 0.5 M NaHPO4, 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at 65° C., and washing in 0.1×SSC/0.1% SDS, wherein the polynucleotide encodes a polypeptide having α1,2-fucosyltranferase activity and catalyzes the synthesis of Lewis Y and Lewis B;
- (d) a polynucleotide comprising a nucleic acid sequences complementary to (i) SEQ ID NO:
- 1, (ii) SEQ ID NO:
- 1, wherein “T” in SEQ ID NO:
- 1 is replaced with “U”, (iii) nucleotides 137-1036 of SEQ ID NO:1, or (iv) nucleotides 137-1036 of SEQ ID NO:
- 1, wherein “T” in the sequence is replace with “U”.
An isolated polynucleotide that hybridizes to a nucleic acid molecule comprising a sequence as set forth in SEQ ID NO:
- 1 under stringent conditions wherein said stringent conditions comprise hybridization at 68° C. in 0.5 M NaHPO4, 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at 65° C., and washing in 0.1×SSC/0.1% SDS, wherein the polynucleotide encodes a polypeptide having α1,2-fucosyltranferase activity and catalyzes the synthesis of Lewis Y and Lewis B.
Dependencies are inferred from the claim text.