US 6,670,124 B1 · High throughput methods of HLA typing

Abstract

A method for determining an HLA genotype of a subject is disclosed. The method comprises (a) isolating template nucleic acid from the subject; (b) amplifying the template nucleic acid to generate sufficient product for each allele of at least one gene locus to be determined; (c) hybridizing the template nucleic acid with an immobilized array of capture oligonucleotides, each having a known nucleic acid sequence of an HLA allele; and (d) determining the particular capture oligonucleotide to which the template nucleic acid hybridizes, thereby determining the genotype of the subject. A number of additional methods that can eliminate or abbreviate additional steps are also described. Moreover, the present invention provides a method for determining tissue compatibility using the determined HLA genotype.

Claims8

Dependent claims

A method for identifying an HLA genotype of a subject, the method comprising:

(a) obtaining a sample comprising a template nucleic acid from said subject;
(b) amplifying said template nucleic acid with a plurality of HLA allele-specific forward primers and HLA allele-specific reverse primers to form amplification products, wherein said forward primers or reverse primers comprise a detectable label;
(c) hybridizing said amplification products with an HLA locus-specific capture oligonucleotide immobilized on a solid phase to form a plurality of detectable complexes, wherein the HLA locus-specific capture oligonucleotide comprises SEQ ID NO:277;
and
(d) detecting said detectable complexes to identify said HLA genotype of said subject.

2 more elements

Dependencies are inferred from the claim text.

High throughput methods of HLA typing

Life of this patent

High throughput methods of HLA typing

Life of this patent

Abstract

Claims8