Methods for the detection, quantification and differentiation of infectious versus non-infectious pathogens in a sample
AssigneeBaxter Aktiengesellschaft
InventorsKlaus Zimmermann, Dirk Völkel, Peter Turecek, Hans-Peter Schwarz, Manfred Rieger
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Abstract
Methods are disclosed that permit pathogenic agent detection and differentiation between infectious and non-infectious forms. Specifically, the present invention provides for pathogenic agent detection using nucleic acid amplification and detection techniques. Moreover, these nucleic acid amplification and detection techniques permit infectious pathogens to be distinguished from non-infectious forms. Also disclosed is a method used to detect and distinguishes between infectious and non-infectious pathogens in biological products and pharmaceutical preparations.
Claims25
A method far determining the type of target nucleic acids in a sample, wherein the method is capable of differentiating free and encapsulated target nucleic acids in the sample, wherein the method comprises
- (a) determining a total target nucleic acid content in the sample;
- (b) adding a nuolease to the sample to digest free target nucleic acids in the sample to form a nuclease-treated sample;
- (c) determining a total target nucleic acid content remaining in the nuctease-treated sample, thereby quantifying the amount of encapsulated target nucleic acids in the sample;
- and
- (d) quantifying the total amount of free target nucleic acid in the sample by subtracting the determined amount of target nucleic acid content in the nuclease-treated sample from the determined amount of total target nucleic acid content in the sample, wherein steps (c) and (d) determine the types of target nucleic acids in the sample;
- differentiating free and encapsulated target nucleic acids in the sample, wherein the method comprises
- (a) determining a total target nucleic acid content in the sample;
- (b) adding a nuclease to the sample to digest free target nucleic acids in the sample to form a nuclease-treated, wherein the nuclease will not digest the encapsulated target nucleic acids;
- (c) determining a total target nucleic acid content remaining undigested in the nuclease-treated sample, which represents the amount of infectious pathogens in the sample;
- (d) quantifying the total amount of free target nucleic acid in the sample by subtracting the determined amount of undigested target nucleic acid content in the nuclease-treated sample from the determined amount of total target nucleic acid content in the sample, wherein the quantifying indicates the amount of inactivated pathogens in the sample;
- and
- (e) comparing the amounts from steps (c) and (d) to determine the proportion of infectious pathogens and inactivated pathogens in the sample.
A method for determining the proportions of infectious pathogens and inactivated pathogens in a sample, wherein the method is capable of differentiating free and encapsulated target nucleic acids in the sample, wherein the method comprises
- (a) determining a total target nucleic acid content in the sample;
- (b) adding a nuclease to the sample to digest free target nucleic acids in the sample to form a digested sample, wherein the nuclease will not digest the encapsulated target nucleic acids;
- (c) determining a total target nucleic acid content remaining in the digested sample, which represents the amount of infectious pathogens in the sample;
- (d) quantifying the total amount of free target nucleic acid in the sample by subtracting the determined amount of target nucleic acid content in the digested sample from the determined amount of total target nucleic acid content in the sample, wherein the quantifying indicates the amount of inactivated pathogens in the sample.
A method for detecting infectious pathogens in a sample, wherein the method comprises
- (a) determining a total target nucleic acid content in the sample;
- (b) adding a maclease to the sample to digest any free target nucleic acids in the sample to form a digested sample, wherein the nuclease will not digest the encapsulated target nucleic acids;
- and
- (c) determining a total target nucleic acid content remaining in the digested sample, which represents the amount of infectious pathogens in the sample.
Dependencies are inferred from the claim text.