Method and apparatus for analyzing organic macromolecular component and application thereof

An organic macromolecular component analysis method comprising the following steps:

• providing a measurement system including a sample introduction section, a preparation section, and a measuring section;
• introducing a sample including an organic macromolecular component into the introduction section;
• transferring the sample to the preparation section;
• a step for separating the organic macromolecular component from the sample;
• and
• moving the separated organic macromolecular component to the measuring section, wherein
• the step for separating comprises the following steps:
• a step for adsorbing the organic macromolecular component on a resin in the preparation section;
• a step for feeding an eluant solution into the preparation section;
• and
• a step for eluting the organic macromolecular component adsorbed on the resin using the eluant solution;
• and further comprising
• introducing a mixture of the eluant solution and a phosphoric acid buffer solution into a detecting device in the measuring section wherein the phosphoric acid buffer solution is introduced into the detecting device at a flow rate of 1 mL/min or less.

An apparatus for flow analysis of an organic macromolecular component in a sample, the apparatus comprising a sample introduction section, a preparation section, and a measuring section, wherein

• the introduction section is connected to the preparation section;
• the preparation section is connected to the measuring section;
• the preparation section comprises a separation device to separate the organic macromolecular component from the sample;
• and
• the measuring section comprises an measurement device to fractionate and analyze the separated organic macromolecular component and a detecting device;
• wherein a mixture of an eluant solution and a phosphoric acid buffer solution is introduced into the detecting device of the measuring section wherein the phosphoric acid buffer solution is introduced into the detecting device at a flow rate of 1 mL/min or less.

An apparatus for flow analysis of an organic macromolecular component in a sample, the apparatus comprising a sample introduction section, a preparation section, and a measuring section, wherein

• the introduction section is connected to the preparation section;
• the preparation section is connected to the measuring section;
• the preparation section comprises a means for separating the organic macromolecular component from the sample;
• and
• the measuring section comprises a means for fractionating and analyzing the separated organic macromolecular component and a detecting device;
• wherein a mixture of an eluant solution and a phosphoric acid buffer solution is introduced into the detecting device of the measuring section wherein the phosphoric acid buffer solution is introduced into the detecting device at a flow rate of 1 mL/min or less.