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US 6,665,072 B2Granted
Instantaneous dual band fluorescence detection systems
AssigneeCambridge Research & Instrumentation, Inc.
InventorClifford C. Hoyt
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Abstract
An apparatus for performing fluorescence detection of two or more biochemical probes and/or fluorescence measurement of fluorescence intensity at two or more spectral bands of light emitted from at least one sample spot is disclosed. The apparatus simultaneously directs emitted fluorescent light from multiple probes and/or at multiple spectral bands to different spots on a single pixelated detector.
Claims34
An apparatus for at least one of fluorescence detection and fluorescence measurement of fluorescence intensity, comprising:
- at least one light emitting source for emitting at least one beam of light for illuminating at least one sample for effecting emissions of fluorescent light from said at least one sample, said emitted fluorescent light comprised at least of first and second predetermined spectral bands;
- and
- a detector positioned for receiving the emitted fluorescent light from the at least one sample, for spatially separating the emitted fluorescent light into component beams based at least on spectral band, and for detecting an emission flux of at least one component beam, said detector, comprising:
- a polarization-sensitive optical element for receiving the emitted fluorescent light, for transmitting the received light in the tint predetermined spectral band therethrough without alteration, and for transmitting the received light in the second predetermined spectral band therethrough such that its polarization state upon exiting the polarization-sensitive optical element is the complement of its polarization state before entering the polarization-sensitive optical element;
- a double refractive element positioned in optical series either before or after the polarization-sensitive optical element, said double refractive element for separating light incident thereon into spatially distinct beams according to polarization state, wherein
- if positioned before the polarization-sensitive optical element, said double-refractive element receives the emitted fluorescent light and spatially separates the received light into two beams according to polarization state;
- or
- if positioned after the polarization-sensitive optical element, said double-refractive element spatially separates the component beams of each set according to polarization state;
- and
- a photodetector for detecting the emission flux of at least one component beam received after passing through both said polarization-sensitive element and said double refractive element.
7 more elements
An apparatus for at least one of fluorescence detection and fluorescence measurement of fluorescence intensity, comprising:
- at least one light emitting source for emitting at least one beam of light for illuminating at least one sample for effecting emissions of fluorescent light from said at least one sample, said emitted fluorescent light comprised at least of first and second predetermined spectral bands;
- and
- a detector positioned for receiving the emitted fluorescent light from the at least one sample, for spatially separating the emitted fluorescent light into four component beams based on spectral band and polarization state, and for detecting an emission flux of each of the four component beams, said detector comprising, in optical series:
- a first double refractive element for spatially separating the emitted fluorescent light into two spatially distinct beams according to polarization state;
- a polarization-sensitive optical element for receiving the two spatially distinct beams and decomposing each of them into a set of two component beams, one component beam carrying the first predetermined spectral band and the other component beam carrying the second predetermined spectral band, wherein the two component beams have orthogonal polarization states but share the same optical path;
- a second double refractive element for receiving the two sets of component beams and for spatially separating the component beams of each set according to polarization state;
- and
- a photodetector for receiving the four spatially distinct component beams and for detecting an emission flux of each.
5 more elements
Dependencies are inferred from the claim text.