Method and device for detecting variations of optical properties in a sample in and analysis process
AssigneeStago International
InventorsKhaled Abou-Saleh, Patrick Fere, Alain Rousseau
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Abstract
The invention concerns a method which consists in performing for a time interval ranging between 1 ms and 10 ms a first turbidimetric measurement using a first light pulse originating from a first source (SL1) emitting in a first frequency range, a second measurement of intensity transmitted in response to a second light pulse originating from a second source (SL2) emitting in a second frequency range and, optionally determining the fluorescence of the sample. The invention is applicable to a biological analysis process as well as to hemostatic analyses.
Claims14
Method for detecting a variation of the optical properties of a sample to be analysed,
- said method consisting of carrying out during a relatively short period of time of about one millisecond to about ten milliseconds an operational sequence comprising at least the following stages:
- i. a first exposure of the sample to a first incident light pulse whose light waves are included in a first range of frequencies, this pulse being emitted by a first opto-electronic light source,
- ii. a first turbidimetric measurement by an opto-electronic detector situated approximately in the axis of said first incident pulse of the light intensity transmitted from said first pulse after passing through said sample,
- iii. a storage of the result of this first measurement in a memory,
- iv. an exposure of the sample to a second incident light pulse having approximately the same incidence as first and whose light waves are included in a second frequency range, this pulse being emitted by a second opto-electronic light source,
- v. a second measurement of the intensity transmitted from said second pulse after passing through said sample,
- vi. a storage of the result of this second measurement in said memory,
- vii. an exposure of the sample to a third light pulse emitted by a third opto-electronic light source orientated perpendicular to the said first and second opto-electronic light sources,
- vii a third nephelometric measurement by said detector,
- viii. a storage of the result of the third measurement in said memory,
- ix. an analysis of the results stored in said memory.
Device for detecting a variation of the optical properties of a sample to be analysed, said device comprising:
- i. a first opto-electronic light source emitting in a first wavelength range a light beam applied by means of a first optical guide to a transmission head axed towards a transparent receptacle containing a sample to be analysed,
- a second light source emitting inside a second wavelength range a light beam applied to said transmission head,
- ii. at least one third light source connected by means of a third optical guide to a transmission head centered on the transparent receptacle perpendicular to the axis of said first transmission head,
- at least one opto-electronic detector placed in said axis at a location situated on the side of the receptacle situated opposite said transmission head so as to give an information about an intensity of said first light bean after passing through said sample,
- a microprocessor comprising:
- i. means for controlling said first opto-electronic light source so as to expose said sample to a first incident light pulse whose light waves are included in a first range of frequencies,
- ii. means for storing the turbidimetric measurement provided by said opto-electronic detector in response to said first incident light pulse,
- iii. means for controlling said second opto-electronic light source so as to expose said sample to a second incident light pulse having approximately the same incidence as the first and whose light waves are included in a second frequency range,
- iv. means for storing the second measurement provided by said opto-electronic detector in response to said second incident light pulse,
- v. means for controlling said third opto-electronic light source so as to expose said sample to a third light pulse,
- vi. means for storing the third nephelometric measurement provided by said opto-electronic detector in response to said third incident light pulse,
- vii. an analysis of the results stored in said memory.
Dependencies are inferred from the claim text.