Plant amino acid biosynthetic enzymes
AssigneeE. I. Du Pont de Nemours and Company
InventorsSaverio Carl Falco, Stephen M. Allen
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyze steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.
Claims10
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having S-adenosylmethionine synthetase activity, wherein the nucleotide sequence encoding the polypeptide and the nucleotide sequence of SEQ ID NO:35 have at least 95% sequence identity based on BLASTN alignment, or
- (b) the complement of the nucleotide sequence encoding the polypeptide, wherein the complement and the nucleotide sequence encoding the polypeptide contain the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.