Transgenic plants presenting a modified inulin producing profile

A recombinant DNA construct, recombinant gene or vector comprising the cDNA sequence according to SEQ ID NO:

• 1.

A recombinant DNA construct, recombinant gene or vector comprising more than one copy of the cDNA sequence according to SEQ ID NO:

• 1.

A method for producing a transgenic plant with a modified inulin producing profile comprising in its genome a combination of a 1-SST (sucrose:sucrose 1-fructosyl-transferase) enzyme encoding gene and a 1-FFT (fructan:fructan 1-fructosyl-transferase) enzyme encoding gene, said method comprising transforming an inulin producing host plant comprising in its genome a combination of a 1-SST enzyme encoding gene and a 1-FFT enzyme encoding gene, by insertion of a recombinant gene containing the 1-SST enzyme encoding a33 cDNA sequence according to SEQ ID NO:

• 1 into the genome of the host plant and wherein said 1-SST enzyme encoding gene, said 1-FFT enzyme encoding gene and said recombinant gene containing the 1-SST enzyme encoding a33 cDNA sequence according to SEQ ID NO:
• 1, are respectively operably linked to a promoter sequence, a terminator sequence and optionally to a DNA sequence encoding a targeting signal or a transit peptide, all active in said host plant.

A method for producing a transgenic plant with a modified inulin producing profile comprising in its genome a combination of a recombinant 1-SST enzyme encoding gene comprising the 1-SST enzyme encoding a33 cDNA sequence according to SEQ ID NO:

• 1, and a recombinant 1-FFT enzyme encoding gene, said method comprising transforming a non-inulin producing host plant by insertion into the genome of the host plant of a recombinant 1-SST enzyme encoding gene comprising the 1-SST enzyme encoding a33 cDNA sequence according to SEQ ID NO:
• 1 and of a recombinant 1-FFT enzyme encoding gene, wherein the recombinant 1-SST enzyme encoding gene and the recombinant 1-FFT enzyme encoding gene are respectively operably linked to a promoter sequence, a terminator sequence and optionally a DNA sequence encoding a targeting signal or a transit peptide, all active in said host plant.

A method for producing a transgenic plant with a modified inulin producing profile comprising in its genome a recombinant gene containing the a33 cDNA sequence according to SEQ ID NO:

• 1 coding for an enzyme which acts as a 1-SST enzyme as well as a 1-FFT enzyme, said method comprising transforming a host plant which is free of 1-FFT enzyme encoding genes by insertion into the genome of the host plant of a recombinant gene containing the a33 cDNA sequence according to SEQ ID NO:
• 1 which is operably linked to a promoter sequence, a terminator sequence and optionally a DNA sequence encoding a targeting signal or a transit peptide, all active in said host plant.

A recombinant gene or vector each comprising a combination of a 1-SST enzyme encoding gene comprising the cDNA sequence according to SEQ ID NO:

• 1, and of a 1-FFT enzyme encoding gene of plant origin, which enzyme encoding genes are respectively operably linked to a promoter sequence and a terminator sequence, and optionally to a DNA sequence encoding a targeting signal or a transit peptide, all active in an host plant.

A transgenic plant with a modified inulin producing profile;

• or a root, shoot, plant part, plant tissue, plant cell thereof or seed thereof, each with a genome according to that of said transgenic plant;
• comprising in its genome a recombinant 1-SST enzyme encoding gene comprising at least one copy of the cDNA sequence according to SEQ ID NO:
• 1, operably linked to a promoter sequence and a terminator sequence.