Pathogenic Tau mutations in transgenic mice
AssigneeMayo Foundation for Medical Education and Research
InventorsMichael L. Hutton, Jada M. Lewis, Eileen M. McGowan
Life of this patent
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Abstract
Isolated nucleic acid molecules that include a tau gene sequence are described. The tau gene sequences have a mutation linked to a Tau pathology. Transgenic non-human mammals that develop a Tau pathology also are described.
Claims17
A transgenic mouse comprising a nucleic acid construct, said construct comprising a regulatory element operably linked to a nucleic acid molecule encoding a human Tau polypeptide, wherein said polypeptide comprises a pathogenic Tau mutation at amino acid 301 and is expressed in the brain of said transgenic mouse, and wherein said transgenic mouse exhibits a Tau pathology.
A transgenic mouse comprising:
- (a) a first nucleic acid construct comprising a regulatory element operably linked to a nucleic acid molecule encoding a human Tau polypeptide, wherein said polypeptide comprises a pathogenic Tau mutation at amino acid 301 and is expressed in the brain of said transgenic mouse;
- and
- (b) a second nucleic acid construct comprising a regulatory element operably linked to a nucleic acid molecule encoding a human amyloid precursor protein (APP), wherein said human APP comprises a mutation at amino acids 670 and 671, and wherein said transgenic mouse exhibits a Tau pathology.
A method for identifying agents that inhibit development of a Tau pathology, said method comprising administering a test agent to a transgenic mouse, said transgenic mouse comprising a nucleic acid construct, said construct comprising a regulatory element operably linked to a nucleic acid molecule encoding a human Tau polypeptide, wherein said polypeptide comprises a pathogenic Tau mutation at amino acid 301 and is expressed in the brain of said transgenic mouse, and wherein said transgenic mouse exhibits a Tau pathology, and determining if said test agent inhibits development of said Tau pathology in said transgenic mouse as compared with a corresponding transgenic mouse to which said test agent has not been administered.
Dependencies are inferred from the claim text.