System for efficient secretion of recombinant proteins
AssigneeThe Research Foundation of State University of New York
InventorsJorge E. Galan, Wolf-Dietrich Hardt
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Abstract
Provided is a signal peptide comprising an amino acid sequence at least 90% homologous to the amino acid sequence as shown in SEQ ID NO:1. Further provided is a fusion protein comprising the signal peptide fused to a heterologous protein. Also provided are nucleic acid molecules encoding the signal peptide and encoding the fusion protein, as well as vectors and recombinant host cells comprising the nucleic acid molecules. The recombinant host cell can be a recombinant bacterium having a functional type III secretion system and having loss-of-function mutations in genes that encode secreted substrate proteins of the type III secretion system. The recombinant host cell can be used in a method of producing the heterologous protein.
Claims17
An isolated nucleic acid molecule encoding a signal peptide having an amino acid sequence consisting of SEQ ID NO:1.
An isolated nucleic acid molecule encoding a fusion protein, the fusion protein comprising a signal peptide fused to a heterologous protein, wherein the signal peptide has an amino acid sequence consisting of SEQ ID NO:1.
An isolated nucleic acid molecule encoding a signal peptide having an amino acid sequence less than 100 amino acids in length and comprising SEQ ID NO:1.
An isolated nucleic acid molecule encoding a fusion protein, the fusion protein comprising a signal peptide fused to a heterologous protein, wherein the signal peptide has an amino acid sequence less than 100 amino acids in length and comprises SEQ ID NO:1.
Dependencies are inferred from the claim text.