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US 6,664,097 B2Granted
Polynucleotide encoding a Lactobacillus gasseri beta-glucuronidase polypeptide
AssigneeNorth Carolina State University
InventorsWilliam Michael Russell, Todd Robert Klaenhammer
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Abstract
The present invention provides isolated β-Glucuronidase (GUS) having activity at acidic pH and nucleic acids encoding the same. The nucleic acids may be isolated from any suitable species, and in a preferable embodiment are isolated from Lactobacillus gasseri.
Claims17
An isolated nucleic acid encoding β-glucuronidase (GUS), said isolated nucleic acid selected from the group consisting of:
- (a) a DNA having the nucleotide sequence given herein as SEQ ID NO:1;
- (b) a polynucleotide that hybridizes to the DNA of (a) above under stringent conditions represented by a wash stringency of 50% Formamide with 5×Denhardt's solution, 0.5% SDS and 1×SSPE at 42° C., and which encodes a β-glucuronidase (GUS) protein;
- and
- (c) a polynucleotide that differs from the DNA of (a) or the polynucleotide of (b) above due to the degeneracy of the genetic code, and which encodes the protein encoded by the DNA of (a) or the polynucleotide of (b) above.
1 more element
Dependencies are inferred from the claim text.