Methods for improved diagnosis and treatment of mycobacterial infections
OwnerYing Zhanginventor
Life of this patent
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Abstract
Media for growth enhancement and resuscitation of mycobacteria (such as Mycobacterium tuberculosis, Mycobacterium paratuberculosis, and Mycobacterium leprae) are provided. The media comprise isolated cell extract, early-stationary-phase or stationary phase supernatant, or a substantially purified component thereof such as a protein, a peptide fragment of the protein, or a phospholipid. The protein is Rv1147c and the phospholipid or a component of a phospholipid. Diagnostic methods and kits utilizing the media are also provided, as well as treatment methods utilizing spent culture supernatant and cell extracts, or components thereof.
Claims57
A supplemented medium for culturing mycobacteria, comprising
- a cell extract from Mycobacterium tuberculosis complex or at least one substantially purified product from Mycobacterium tuberculosis complex, wherein said cell extract or said substantially purified product exhibits growth enhancement activity for said mycobacteria, initiation of growth for small inocula, or resuscitation activity for dormant mycobacterial bacilli, and
- a suitable culture medium.
A method for reviving dormant bacilli of a mycobacterium species, comprising the step of
- exposing said dormant bacilli of said mycobacterium species to cell extract of Mycobacterium tuberculosis complex or at least one substantially purified product of Mycobacterium tuberculosis complex, wherein said cell extract or said substantially purified product exhibits growth enhancement and resuscitation activity for mycobacteria, and wherein said substantially purified product is present in sufficient quantity to effect revival of said dormant bacilli of said mycobacterium species.
A method for the diagnosis of infection caused by a mycobacterium species, comprising,
- combining a sample for which the presence or absence of said mycobacterium species is to be determined with medium supplemented with cell extract of Mycobacterium tuberculosis complex or at least one substantially purified product of Mycobacterium tuberculosis complex in a culture, wherein said cell extract or said substantially purified product exhibits growth enhancement and resuscitation activity for mycobacteria;
- and
- analyzing said culture for the presence of said mycobacterium species, wherein a finding of the presence of said mycobacterium species indicates a positive diagnosis for said infection.
A kit for the diagnosis of infection caused by a mycobacterium species, comprising
- a sealed container of medium supplemented with cell extract of Mycobacterium tuberculosis complex or at least one substantially purified product of Mycobacterium tuberculosis complex, wherein said substantially purified product exhibits growth enhancement and resuscitation activity for mycobacteria, in a carrier fluid.
A pharmacological agent for the treatment of an infection caused by a mycobacterium species, comprising,
- cell extract of Mycobacterium tuberculosis complex or at least one substantially purified product of Mycobacterium tuberculosis complex, wherein said substantially purified product exhibits growth enhancement and resuscitation activity for mycobacteria, and
- a physiologically suitable carrier.
A culture medium for culturing a mycobacterium species, comprising
- isolated early-stationary-phase or stationary phase supernatant of Mycobacterium tuberculosis complex.
A method for reviving dormant bacilli of a mycobacterium species, comprising the step of
- exposing said dormant bacilli of said mycobacterium species to isolated early-stationary-phase or stationary phase supernatant of Mycobacterium tuberculosis complex, wherein said step of exposing causes revival of said dormant bacilli.
A kit for the diagnosis of infection caused by a mycobacterium species, comprising
- a sealed container comprising isolated early-stationary-phase or stationary phase supernatant from a culture of Mycobacterium tuberculosis complex.
A method for the diagnosis of infection caused by a mycobacterium species comprising,
- combining a sample for which the presence or absence of said mycobacterium species is to be determined with isolated early-stationary-phase or stationary phase supernatant of Mycobacterium tuberculosis complex in a culture;
- and
- analyzing said culture for the presence of said mycobacterium species, wherein a finding of the presence of said mycobacterium species indicates a positive diagnosis for said infection.
A method of inhibiting the growth of a species of mycobacterium comprising the step of
- exposing said mycobacterium an isolated supernatant or cell extract from a culture of Mycobacterium tuberculosis complex that is at least 3 months old, wherein said step of exposing has the effect of inhibiting the growth of said species of mycobacterium.
A method for enhancing the growth of a mycobacterium species, comprising,
- culturing said mycobacterium species in a culture medium supplemented with cell extract of Mycobacterium tuberculosis complex or at least one substantially purified product of Mycobacterium tuberculosis complex, wherein said cell extract or said substantially purified product exhibits growth enhancement for said mycobacterium species.
Dependencies are inferred from the claim text.