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AssigneeRoche Vitamins Inc.
InventorsTatsuo Hoshino, Takahide Kon, Masako Shinjoh, Masaaki Tazoe
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The present invention provides a genetically engineered microorganism belonging to the genus Gluconobacter or Acetobacter, which has an engineered gene for the biological activity of reducing L-sorbose which is more than 90% non-functional in developing the said biological activity. The engineered microorganism is derived from a microorganism belonging to the genus Gluconobacter or Acetobacter, and has the biological activity for reducing L-sorbose that is less than 10% of the amount of the activity of the wild type organism. The present invention further provides a method for producing the genetically engineered microorganism by mutating the gene of a microorganism of the genus Gluconobacter or Acetobacter which encodes a protein having the L-sorbose reductase activity, determining the L-sorbose reductase activity of the resulting microorganism with the mutated gene and selecting the genetically engineered microorganism having L-sorbose reductase activity that is less than 10% of the amount of the activity of the wild type microorganism.
A genetically engineered microorganism obtained from a microorganism belonging to the genus Gluconobacter, said engineered microorganism comprising a mutated polynucleotide having a mutation in a polynucleotide selected from the group consisting of SEQ ID NO:1, a fragment of SEQ ID NO:1 encoding a polypeptide having L-sorbose reductase activity and a polynucleotide encoding SEQ ID NO:2, wherein the mutation results in a biological activity for reducing L-sorbose which is less than 10% of the amount of the activity of the non-mutated Gluconobacter microorganism.
A process for producing a genetically engineered microorganism comprising:
Dependencies are inferred from the claim text.