Nucleic acid amplification and detection of mycobacterium species
AssigneesGen-Probe Incorporated, Bio Merieux
InventorsSteven T. Brentano, Markus T. Jucker, Francisco D. Delgado, Philippe Cleuziat, Marc Rodrigue
Life of this patent
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Abstract
Methods of detecting Mycobacterium species using oligonucleotides to amplify in vitro 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species in the genus Mycobacterium are disclosed. Amplification oligonucleotides for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed. Kits containing oligonucleotides useful for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed.
Claims16
A composition comprising a combination of oligonucleotides for amplifying in an in vitro amplification reaction a Mycobacterium 16S rRNA sequence or DNA encoding the Mycobacterium 16S rRNA, wherein the oligonucleotides are:
- an oligonucleotide in a size range of about 21 to 26 bases containing a base sequence consisting of at least 18 contiguous bases contained in SEQ ID NO:5, and
- an oligonucleotide in a size range of about 18 to 26 bases containing a base sequence consisting of at least 18 contiguous bases contained in SEQ ID NO:34.
A kit comprising a combination of oligonucleotides wherein the oligonucleotides are:
- an oligonucleotide in a size range of about 21 to 26 bases containing a base sequence consisting of at least 18 contiguous bases contained in SEQ ID NO:5, and
- an oligonucleotide in a size range of about 18 to 26 bases containing a base sequence consisting of at least 18 contiguous bases contained in SEQ ID NO:34.
A method of detecting Mycobacterium species present in a biological sample, comprising the steps of:
- providing a biological sample containing nucleic acid from at least one Mycobacterium species comprising a Mycobacterium 16S ribosomal RNA (rRNA) or a DNA encoding the Mycobacterium 16S ribosomal rRNA;
- amplifying the Mycobacterium 16S rRNA or DNA in an in vitro nucleic acid amplification mixture comprising at least one polymerase activity, and a combination of at least two primers, to produce amplified Mycobacterium nucleic acid, wherein the primers are an oligonucleotide in a size range of about 21 to 26 bases containing a base sequence consisting of at least 18 contiguous bases contained in SEQ ID NO:5, and an oligonucleotide in a size range of about 18 to 26 bases containing a base sequence consisting of at least 18 contiguous bases contained in SEQ ID NO:34;
- and
- detecting the amplified Mycobacterium nucleic acid by detecting a label associated with the amplified Mycobacterium nucleic acid.
Dependencies are inferred from the claim text.