Modification of bacteria
AssigneeElsworth Biotechnology Limited
InventorsEdward M. Green, Fiona S. Cusdin, Namdar Baghaei-Yazdi, Muhammad Javed
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Abstract
Sporulation-deficient variants of thermophilic, facultatively anaerobic, Gram-positive bacteria can be generated using a plasmid transformation system based on a novel method of in vivo methylation. Such bacteria exhibit improved ethanol production-related characteristics.
Claims16
A method of producing a recombinant thermophilic Bacillus sp comprising in vivo methylation in a host cell by a non-endogenous DNA methylase of a heterologous gene and introduction of that methylated heterologous gene into the Bacillus, wherein the Bacillus is capable of growth at a temperature greater than 60° C.
A thermophilic Bacillus sp which has been transformed with a methylated heterologous gene, wherein the Bacillus is capable of growth at a temperature greater than 60° C.
The plasmid pUBUC as illustrated in FIG. 1.
The plasmid pMETH as illustrated in FIG. 2.
Dependencies are inferred from the claim text.