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US 6,664,063 B1Granted
Methods for separating,fractionating and analyzing gene of virus existing in hydrosphere
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsAkihiko Maruyama, Hisaaki Yagi, Ryuichiro Kurane
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Abstract
A method for separating genes from viruses existing in hydrosphere in an intact state, a method for fractionating thus separated genes, and a method for analyzing thus fractionated genes.
Claims15
A method for separating virus genes, comprising:
- (a) separating virus particles from hydrosphere;
- (b) embedding the virus particles obtained in (a) in a gel;
- and
- (c) treating the embedded product obtained in (b) with a proteolytic enzyme and/or a surfactant.
1 more element
A method for analyzing virus genes, comprising:
- (a) separating virus particles from hydrosphere;
- (b) embedding the virus particles obtained in (a) in a gel;
- (c) treating the embedded product obtained in (b) with a proteolytic enzyme and/or a surfactant;
- and
- (d) subjecting the proteolytic enzyme- and/or sufactant-treated product obtained in (c) to electrophoresis.
2 more elements
A method for analyzing virus genes, comprising:
- (a) separating virus particles from hydrosphere;
- (b) embedding the virus particles obtained in (a) in a gel;
- (c) treating the embedded product obtained in (b) with a proteolytic enzyme and/or a surfactant;
- (d) subjecting the proteolytic enzyme- and/or sufactant-treated product obtained in (c) to electrophoresis;
- and
- (e) analyzing the electrophoresis gel obtained in step (d).
3 more elements
Dependencies are inferred from the claim text.