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US 6,664,061 B2Granted
Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
AssigneeAmersham Biosciences AB
InventorsRobert Elghanian, Charles K. Brush, Yanzheng Xu
Life of this patent
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Abstract
[2+2] photo-attachable functional groups were incorporated in polyacrylamide based hydrogels and synthetic oligonucleotide probes. The probes were photochemically attached by covalent bonding to the three dimensional surface of a hydrogel.
Claims94
A method of performing an expression microarray to determine the presence of a target, comprising:
- (a) attaching a probe which will recognize a target to a polymer-coated support by a [2+2] photocycloaddition to form a microarray;
- (b) contacting an aqueous target solution with the microarray, for a time sufficient to form a complex between complementing targets and probes, wherein the target solution comprises an aqueous buffer solution and the target;
- and
- (c) scanning the microarray to determine the presence of the target.
1 more element
A method of performing a single nucleotide polymorphism microarray to determine the presence of a target, comprising:
- (a) attaching a probe which will recognize a target to a polymer-coated support by a [2+2] photocycloaddition to form a microarray;
- (b) contacting an aqueous target solution with the microarray, for a time sufficient to form a complex between complementing targets and probes, wherein the target solution comprises an aqueous buffer solution, the target, an active enzyme, and a labeled carrier;
- and
- (c) scanning the microarray to determine the presence of the target.
1 more element
Dependencies are inferred from the claim text.