US 6,664,057 B2 · Amplicon in the 20q13 region of human chromosome 20 and uses thereof

Abstract

This invention pertains to the field of cancer genetics and cytogenetics. In particular, this invention pertains to the identification of a novel amplicon on human chromosome 20 which is associated with cancer. More particularly this invention pertains to the identification of a novel “amplicon” or genomic nucleic acid in a region of amplification at about 20q13.2 which has been associated with a variety of cancers, particularly breast cancer. The novel amplicon of the invention can be used as a probe specific for this region of 20q13.2 as well as for the diagnosis and prognosis of various cancers. Also provided are kits for screening for the presence and copy number of the novel amplicon of the invention in a sample containing human nucleic acid.

Claims16

Dependent claims

A method for screening for the presence or absence of an amplicon in a sample of human nucleic acid, the method comprising:

providing a sample of nucleic acid derived from a human cell;
providing a probe, wherein the probe hybridizes specifically to a chromosomal region from D20S211 through D20S120 under wash conditions that include 0.2×SSC at 65° C.;
contacting the human nucleic acid with the probe, wherein the probe is contacted with the human nucleic acid under conditions in which the probe binds selectively under stringent conditions to the human nucleic acid to form a hybridization complex;
and
detecting the formation of the hybridization complex to confirm the presence or absence of the amplicon.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/896,070Filed 28 Jun 2001 · division

Priority

14 Aug 1998

Published

US 2002/0120526 A129 Aug 2002

Granted

16 Dec 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6841
Hybridisation assays: in situ hybridisation
C12Q 1/6886
For diseases caused by alterations of genetic material: for cancer
C12Q 2600/156
Oligonucleotides characterized by their use: polymorphic or mutational markers
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Amplicon in the 20q13 region of human chromosome 20 and uses thereof

Life of this patent

Amplicon in the 20q13 region of human chromosome 20 and uses thereof

Life of this patent

Abstract

Claims16