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US 6,664,051 B1Granted
Fixation of nucleotide derivatives to solid carrier
AssigneeFuji Photo Film Co., Ltd.
InventorsHiroshi Shinoki, Yukio Sudo, Osamu Seshimoto
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Abstract
A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.
Claims9
A method for fixing a plurality of nucleotide derivatives to a solid carrier comprising:
- contacting aqueous phase nucleotide derivatives each having a reactive group at one terminal with a solid carrier having thereon reactive groups in the presence of a transferase
- wherein the transferase produces a covalent bond by rearrangement of the reactive group of each nucleotide derivative and the reactive groups of the solid carrier thereby fixing the plurality of nucleotide derivatives to a solid carrier;
- wherein the nucleotide derivatives are selected from the group consisting of oligonucleotides, polynucleotides and peptide-nucleic acids.
Dependencies are inferred from the claim text.