Polyoxime compounds and their preparation
AssigneeGryphon Therapeutics, Inc.
InventorsKeith Rose, Robin E. Offord
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Abstract
A homogeneous polyoxime composition is provided, in which the polyoxime molecules present comprise a first organic baseplate molecule, which is a polypeptide, wherein the baseplate molecule is linked to at least two second organic molecules, which may be the same or different from one another. In the compositions, the linkages between the baseplate and said organic molecules are oxime linkages formed by reaction of an orthogonal reactive group on each the organic molecules with a complementary orthogonal reactive group on the baseplate.
Claims26
A homogeneous polyoxime composition, in which polyoxime molecules present in said composition comprise a baseplate organic molecule, said baseplate organic molecule being a polypeptide, and wherein said baseplate organic molecule is linked to at least two second organic molecules, which may be the same or different from one another, the linkages between said baseplate organic molecule and said second organic molecules being oxime linkages formed by reaction of an orthogonal reactive group on each of said second organic molecules with at least two complementary orthogonal reactive groups on said baseplate organic molecule, said orthogonal reactive group and said complementary orthogonal reactive group being selected from the group consisting of a keto group, an aldehyde group, or an amino-oxy group, and wherein complementary orthogonal reactive group on said baseplate organic molecule comprises an amino acid residue having a side chain comprising at least one of said complementary orthogonal reactive groups.
A homogeneous polyoxime composition prepared by a process comprising the steps of:
- (A) obtaining a baseplate organic molecule, said baseplate organic molecule having present therein a plurality of at least two identical orthogonal reactive groups capable of oxime linkage formation with a complementary keto, aldehyde, or amino-oxy orthogonal reactive group, said baseplate organic molecule comprising an amino acid residue having a side chain comprising at least one of said orthogonal reactive groups;
- (B) obtaining a second organic molecule, said second organic molecule having present therein said complementary orthogonal reactive group capable of oxime linkage formation with said orthogonal reactive group on said baseplate organic molecule;
- (C) contacting said baseplate organic molecule with an amount of said second organic molecule sufficient for complete reaction between said second organic molecule and said plurality of orthogonal reactive groups on said baseplate organic molecule under conditions allowing oxime linkage formation;
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- (D) isolating the polyoxime product.
A hetero-polyoxime composition produced by a process comprising the steps of:
- (A) obtaining a baseplate organic molecule, said baseplate organic molecule having present therein a plurality of at least two orthogonal reactive groups capable of oxime linkage formation with a complementary keto, aldehyde, or amino-oxy orthogonal reactive group, said baseplate organic molecule comprising an amino acid residue having a side chain comprising at least one of said orthogonal reactive groups;
- (B) obtaining a second organic molecule, said second organic molecule having present therein said complementary orthogonal reactive group capable of oxime linkage formation with an orthogonal reactive group on said baseplate organic molecule;
- (C) obtaining a third organic molecule, said third organic molecule having present therein an orthogonal reactive group capable of oxime linkage formation with a complementary orthogonal reactive group on said baseplate organic molecule;
- said third organic molecule being a metal chelating agent, a detectable marker, an imaging agent or a lipophilic anchor;
- (D) mixing said baseplate organic molecule and the second organic molecules under conditions allowing oxime linkage formation;
- (E) mixing said baseplate organic molecule and the third organic molecules, either contemporaneously or sequentially with said mixing in step (D), under conditions allowing oxime linkage formation;
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- (F) isolating the polyoxime product.
Dependencies are inferred from the claim text.