Hybrid heterodimeric protein hormone comprising a TNF binding protein and a glycoprotein hormone subunit and method of using same
AssigneeApplied Research Systems ARS Holding N.V.
InventorsRobert K. Campbell, Bradford A. Jameson, Scott C. Chappel
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Abstract
A hybrid protein includes two coexpressed amino acid sequences forming a dimer. Each sequence contains the binding portion of a receptor, such as TBP1 or TBP2, or a ligand, such as IL-6, IFN-β and TPO, linked to a subunit of a heterodimeric proteinaceous hormone, such as hCG. Each coexpressed sequence contains a corresponding hormone subunit so as to form a heterodimer upon expression. Corresponding DNA molecules, expression vectors and host cells are also disclosed as are pharmaceutical compositions and a method of producing such proteins.
Claims3
A method for inhibiting TNF activity, comprising administering to a patient in need thereof an effective amount of a hybrid protein comprising two different coexpressed amino acid sequences forming a heterodimer, each comprising:
- (a) at least one amino acid sequence selected from the group consisting of TNF Binding Protein 1 (TBP1), TNF Binding Protein 2 (TBP2), and a fragment of said TBP1 or TBP2 still containing the ligand binding domain;
- and
- (b) a subunit of hCG, FSH, LH, TSH, or a fragment of hCG, FSG, LH or TSH which retains the ability of the subunit to form a heterodimer with other subunits thereof;
- wherein sequences (a) and (b) are joined either directly or through a peptide linker, and in which the sequences (b) in each of said two coexpressed sequences aggregate with each other to dimerize and form a heterodimer.
Dependencies are inferred from the claim text.