Method of producing antibodies by immunization with conjugates of molecules coupled to charge-modified proteins

A method for producing antibodies against molecules in a host animal comprising:

• (a) producing a modified carrier protein from unmodified carrier protein wherein the first portion of said unmodified carrier protein is chemically modified to carry a net positive charge and the second portion is chemically modified to carry a net negative charge;
• (b) coupling a molecule to said modified carrier proteins obtained in step (a) to form a complex;
• (c) forming a balanced charge mixture comprising complexes obtained in step (b);
• (d) pre-immunizing a host animal with an unmodified carrier protein;
• (e) administering to the host animal the balanced charge mixture obtained in step (c) so that antibodies are produced against the molecule;
• and
• (f) isolating the antibodies or B cells producing antibodies against the molecule from the host animal.