Cytological stain composition including verification characteristic
AssigneeCytyc Corporation
InventorsDavid J. Zahniser, Norman W. Soule, Louise M. Isenstein
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Abstract
The present invention relates to the analysis of cytological material. Specifically, the invention relates to stains and methods of producing the stains, methods of staining cells for cytological or histological analysis to contrast the nuclear portion of the cell from the cytoplasmic portion, and systems and methods for illuminating a cytological sample. The analysis can be automated or manual.
Claims18
A method for verifying a cytological stain, the method comprising the steps of:
- staining a cytological sample with a stain having a multiple absorption spectrum;
- verifying the stain by illuminating the sample with a light source having a first narrow band wavelength that is substantially absorbed by the stain;
- and
- imaging the sample by illuminating the sample with the light source having a second narrow band wavelength that is substantially absorbed by the stain.
A method for verifying a cytological stain staining a sample, the method comprising the steps of:
- illuminating the sample with a light source having a first wavelength band;
- verifying the stain used based upon light absorption of the sample;
- and
- imaging the sample by illuminating the sample with a light source having a second wavelength band different than the first wavelength band.
A method for verifying a cytological stain staining a sample, the method comprising the steps of:
- verifying the stain by illuminating the sample with a light source having a first narrow band wavelength that is substantially absorbed by the stain;
- and
- imaging the sample by illuminating the sample with the light source having a second narrow band wavelength that is substantially absorbed by the stain.
A method of imaging a specimen stained with a stain having multiple spectral absorption peaks, the method comprising the steps of:
- illuminating the stained specimen with a multiple wavelength light source;
- and
- obtaining an image of the stained specimen, wherein the image is obtained at a narrow band wavelength generally corresponding to a valley formed between two of the multiple spectral absorption peaks of the stain.
Dependencies are inferred from the claim text.